Name: Nebras Rada Mohammed

Nationality: Iraqi

Degree: Lecturer Dr. Doctorate

Address : Iraq/ Baghdad/ Al-Turath University college

Title: Preparation of a new culture medium to stimulate increased production of Staphylokinase produced from Staphylococcus aureus by using Acridine Orange(AO).

أسم الوسط الزرعي الجديد

Nebras Increase Production Staphylokinase Acridine Orange Agar

Summary

Preparation of a new culture medium to stimulate increased production of Staphylokinase produced from Staphylococcus aureus by Acridine Orange(AO).

The study design of cases are Cross-sectional study that descriptive study by internal comparsion of 500 isolates collected from different provenance of human infections including 280(56%) from tonsils , 100(20%) nose, 40(8%) tumors, 17(3.4%) urine, 27(5.4%) skin and 36(7.2%) blood, the isolation and identification achieved by using Vitek2-GP and Genotypic detection PCR to confirmitive identification of S.aureus isolated from tumors.

The results of growth on the new cultural medium when added Acridine Orange(AO) showed there are growth when added 10 g to new culture medium, the growth of bacteria on new culture medium changed the color of bacteria different from the original of S.aureus become very dark and dark green color, also lost its blood hemolysis compared with the original became non hemolysis after growing on the new culture medium, either antibiotics susceptiblity test work after growing on the new culture medium that it became sensitive to Vancomycin, Methicillin, Imipenem, Erythromycin and Oxacillin compared with the original was resistant. The growth on new culture medium it lost resistance to antibiotics and lost the production of the hemolytic enzyme(hemolysis).

The test for the production of the Staphylokinase (Thrombolytic enzyme) on the plasma agar and milk agar medium, it was turn off overproduction to Staphylokinase when grown on new culture medium that Acridine Orange(AO) therefore turn off from harmful bacteria into non-harmful bacteris with overproduction of staphylokinase when added the concentrations 10 gm but no growth when added 20g, 30 g. The bacterium also examined on the new culture medium for having the Sak gene that encoding for Staphylokinase (Fibrinolytic enzyme), the results grant positive result for possess sak gene that encoded for Staphylokinase.